(M1) Multiple Choice. Write the letter of the correct answer on a separate sheet of paper.

11. Below are the steps in recombinant DNA technology. What is the correct sequence of the given steps?

I. ligation (joining) of the gene of interest (eg. from an animal) with the vector (cut bacterial plasmid)
II. cutting or cleavage of DNA by restriction enzymes (REs)
III. transfer of the recombinant plasmid into a host cell
IV. selection process to screen which cells contain the gene of interest

A. I, II, III, IV
B. IV, III, II, I
C. II, IV, I, III
D. II, I, III, IV

12. Which of the following organisms can produce more copies of the recombinant insulin gene in a shorter period?
A. bacteria
B. mouse
C. pig
D. yeast

13. What happens during the denaturation step of a polymerase chain reaction?
A. The DNA nucleotides are broken apart.
B. The DNA is returned to its natural setting.
C. The base-pairing rules for DNA are reversed.
D. The double-stranded DNA is separated into two single strands ofDNA.

14. Which nutrient is highly present in genetically engineered golden rice?
A. collagen
B. omega 3
C. Vitamin A
D. beta-carotene

15. Which of the following steps happened during heat shock treatment?
A. The cell is exposed to an electrical shock.
B. The DNA particle is inserted into the cell through gamma rays.
C. DNA-coated pellets are fired to the plant tissues using a gene gun.
D. The cell is exposed to rapid rise and drop of temperature thereby increasing pore size.