(M1) Multiple Choice. Write the letter of the correct answer on a separate sheet of paper.

6. What enzyme cuts this DNA sequence 5’ ATGAGTTCCCCGAATGTC 3’?
A. EcoR1
B. Hind III
C. Hind I
D. Taq I

7. At what temperature does the denaturation of DNA double helix takes place?
A. 54°C
B. 60°C
C. 74°C
D. 95°C

8. Which enzyme is used to join the bacterial plasmid and the inserted DNA sequence?
A. DNA ligase
B. DNA gyrase
C. DNA helicase
D. DNA polymerase

9. Which of the following procedures occur after the desired gene has been cleaved?
A. expression of the gene
B. insertion to the vector
C. ligation of the gene of interest
D. reproduction of the desired gene

10. What is the function of the antisense RNA inserted in Flavr Savr tomato which has a delayed fruit ripening?
A. activator
B. inhibitor
C. regulator
D. vector