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CaCl2 is known to increase the efficiency of DNA uptake to produce transformed bacterial cells. The divalent Ca2+ ions create transient pores on the bacterial cell wall by which the entry of foreign DNA is facilitated into the bacterial cells.

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ᴄᴀʟᴄɪᴜᴍ ᴄʜʟᴏʀɪᴅᴇ (ᴄᴀᴄʟ2) ᴛʀᴀɴsғᴏʀᴍᴀᴛɪᴏɴ ɪs ᴀ ʟᴀʙᴏʀᴀᴛᴏʀʏ ᴛᴇᴄʜɴɪϙᴜᴇ ɪɴ ᴘʀᴏᴋᴀʀʏᴏᴛɪᴄ (ʙᴀᴄᴛᴇʀɪᴀʟ) ᴄᴇʟʟ ʙɪᴏʟᴏɢʏ. ... ᴛʜᴇ ᴀᴅᴅɪᴛɪᴏɴ ᴏғ ᴄᴀʟᴄɪᴜᴍ ᴄʜʟᴏʀɪᴅᴇ ᴛᴏ ᴀ ᴄᴇʟʟ sᴜsᴘᴇɴsɪᴏɴ ᴘʀᴏᴍᴏᴛᴇs ᴛʜᴇ ʙɪɴᴅɪɴɢ ᴏғ ᴘʟᴀsᴍɪᴅ ᴅɴᴀ ᴛᴏ ʟɪᴘᴏᴘᴏʟʏsᴀᴄᴄʜᴀʀɪᴅᴇs (ʟᴘs).

• ᴡʜᴀᴛ ᴅᴏᴇs ᴄᴀᴄʟ2 ᴅᴏ ɪɴ ᴏʀᴅᴇʀ ᴛᴏ ᴍᴀᴋᴇ ᴄᴇʟʟs ᴄᴏᴍᴘᴇᴛᴇɴᴛ ɪɴ ᴄᴀᴄʟ2 ᴛʀᴇᴀᴛᴍᴇɴᴛ?

ᴄᴀ2+  ʀᴏʟᴇ ɪs ᴛᴏ ɴᴇᴜᴛʀᴀʟɪᴢᴇ ɴᴀᴛᴜʀᴀʟ ɴᴇɢᴀᴛɪᴠᴇ ᴄʜᴀʀɢᴇs ʙᴇᴀʀᴇᴅ ʙᴏᴛʜ ʙʏ ᴅɴᴀ ᴀɴᴅ ᴄᴇʟʟ sᴜʀғᴀᴄᴇ(ᴍᴀɪɴʟʏ ʟᴘs) ᴛᴏ ᴀᴠᴏɪᴅ ᴇʟᴇᴄᴛʀᴏsᴛᴀᴛɪᴄ ʀᴇᴘᴜʟsɪᴏɴ ʙᴇᴛᴡᴇᴇɴ ᴛʜᴇ ᴛᴡᴏ.