Steps involved for the preparation of temporary mount of the stained leaf are given below:
∙ Remove a portion of the peel from the lower surface of a healthy leaf. This can be accomplished by folding the leaf over and gently pulling the peel apart with forceps. Keep the peel in a water-filled watch glass.
∙ In that watch glass, add a few drops of safranin stain. Take out the peel after 2-3 minutes and place it on a clean glass slide.
∙ Apply a drop of glycerin to the peel. Using a needle, gently place a clean coverslip over it. Using blotting paper, remove any excess stain and glycerine
∙ Examine the slide with the compound microscope at various magnifications
Answers & Comments
Answer:
Carefully pull out the thin membranous transparent layer from lower epidermis.
Drop it in the water in a Petridish and add a drop of Safranin stain.
Transfer the stained peel to the clean glass slide and add a drop of glycerine.
4.Cover the material with the cover slip.
Verified answer
Steps involved for the preparation of temporary mount of the stained leaf are given below:
∙ Remove a portion of the peel from the lower surface of a healthy leaf. This can be accomplished by folding the leaf over and gently pulling the peel apart with forceps. Keep the peel in a water-filled watch glass.
∙ In that watch glass, add a few drops of safranin stain. Take out the peel after 2-3 minutes and place it on a clean glass slide.
∙ Apply a drop of glycerin to the peel. Using a needle, gently place a clean coverslip over it. Using blotting paper, remove any excess stain and glycerine
∙ Examine the slide with the compound microscope at various magnifications
hope it helps
and btw thanks for thanking answers