what procedure do you follow to understand the effect of plant growth hormones ( in agar medium ) in the terminal portion of the tip of stem ( coleoptile)
Auxin helps in rapid cell division thereby increasing the stem length. To understand the effect of plant growth hormone in the terminal portion of the tip of the stem, the tip is cut down and then the new branches emerge out from the lateral side of the stem
To show the growth of Avena coleoptile involves a chemical substance.
❖Apparatus:
Oat seedlings (Avena sativa)
coleoptile
a slice of agar
a sharp blade
❖Procedure:
➠ Take some seedlings of coleoptile cut the tips by using a sharp blade.
➠ Place the tips on a slice of agar and leave them for about an hour.
➠ Cut the agar into small blocks and place a block on one side each stump of the decapitated plants.
➠They were kept in the dark during the entire experiment.
➠ Observe the plants after one hour.
❖Observation:
It is observed that a distinct bending away from the side on which the agar block was placed.
Agar block that had not been in contact with coleoptile tip produced either no bending or only a slight bending towards the side on which the block had been placed.
❖Conclusion:
The coleoptile tip exerted its effect by means of a chemical stimulus such as an electrical impulse. This chemical stimulus came to be known as auxin.
Answers & Comments
Answer:
Auxin helps in rapid cell division thereby increasing the stem length. To understand the effect of plant growth hormone in the terminal portion of the tip of the stem, the tip is cut down and then the new branches emerge out from the lateral side of the stem
Explanation:
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❖ Aim :
To show the growth of Avena coleoptile involves a chemical substance.
❖ Apparatus :
❖ Procedure :
➠ Take some seedlings of coleoptile cut the tips by using a sharp blade.
➠ Place the tips on a slice of agar and leave them for about an hour.
➠ Cut the agar into small blocks and place a block on one side each stump of the decapitated plants.
➠They were kept in the dark during the entire experiment.
➠ Observe the plants after one hour.
❖ Observation :
It is observed that a distinct bending away from the side on which the agar block was placed.
Agar block that had not been in contact with coleoptile tip produced either no bending or only a slight bending towards the side on which the block had been placed.
❖ Conclusion :
The coleoptile tip exerted its effect by means of a chemical stimulus such as an electrical impulse. This chemical stimulus came to be known as auxin.
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