Hershey and Chase's famous experiment involved infecting bacteria with bacteriophages (viruses that infect bacteria) to determine whether DNA or protein was the genetic material responsible for controlling the characteristics of the virus.
To differentiate between DNA and protein, Hershey and Chase used radioactive isotopes. They labeled the DNA of one set of bacteriophages with radioactive phosphorus-32 (32P), and the protein of another set of bacteriophages with radioactive sulfur-35 (35S).
They then allowed each set of bacteriophages to infect separate groups of bacteria. After infection, they agitated the bacteria to remove any unattached viral particles. They then separated the bacteriophages from the bacteria, either by blending and centrifuging the mixture or by passing it through a filter, depending on the particular experimental setup.
They found that the bacteria infected with the 32P-labeled bacteriophages became radioactive, indicating that DNA was the genetic material that had been transferred from the virus to the bacteria. In contrast, the bacteria infected with the 35S-labeled bacteriophages did not become radioactive, indicating that protein was not transferred to the bacteria in a way that could lead to the expression of viral traits.
These results supported the hypothesis that DNA, not protein, was the genetic material responsible for the transmission of heritable traits from one generation to the next.
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They used different radioactive isotopes to label DNA and protein coat of the bacteriophage. They grew some bacteriophages on a medium containing radioactive phosphorus ((32)P) to identify DNA and some on a medium containing radioactive sulphur ((35)S) to identify protein
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Answer:
Hershey and Chase's famous experiment involved infecting bacteria with bacteriophages (viruses that infect bacteria) to determine whether DNA or protein was the genetic material responsible for controlling the characteristics of the virus.
To differentiate between DNA and protein, Hershey and Chase used radioactive isotopes. They labeled the DNA of one set of bacteriophages with radioactive phosphorus-32 (32P), and the protein of another set of bacteriophages with radioactive sulfur-35 (35S).
They then allowed each set of bacteriophages to infect separate groups of bacteria. After infection, they agitated the bacteria to remove any unattached viral particles. They then separated the bacteriophages from the bacteria, either by blending and centrifuging the mixture or by passing it through a filter, depending on the particular experimental setup.
They found that the bacteria infected with the 32P-labeled bacteriophages became radioactive, indicating that DNA was the genetic material that had been transferred from the virus to the bacteria. In contrast, the bacteria infected with the 35S-labeled bacteriophages did not become radioactive, indicating that protein was not transferred to the bacteria in a way that could lead to the expression of viral traits.
These results supported the hypothesis that DNA, not protein, was the genetic material responsible for the transmission of heritable traits from one generation to the next.
If you brainliest & follow me, i can help you in future.
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Answer:
They used different radioactive isotopes to label DNA and protein coat of the bacteriophage. They grew some bacteriophages on a medium containing radioactive phosphorus ((32)P) to identify DNA and some on a medium containing radioactive sulphur ((35)S) to identify protein
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