Restriction Enzymes Bam HI Eco RI Hpa I Hind III Nde I Sal I Restriction Enzymes Handout DNA Sequence (Both strands are represented) G GATCC CCTAG G G AATTC CTTAA G GTT AAC CAA TTG A AGCTT TTCGA A CA TATG GTAT AC G TCGAC CAGCT G Restriction enzymes recognize particular sequences in DNA and cut at specific points within that sequence. For example, Bam HI recognizes the DNA sequence "GGATCC". It then cuts between the G and the G. Remember, the DNA is double stranded. The restriction enzymes will cut both strands. Therefore, Bam HI will cut between the Gs on both strands creating "sticky ends." Hpa I cut creating "blunt ends".​