Directions: Arrange chronologically the following steps on the basic microscopy. Write A for the first
step, B for the second, C for the third, D for the fourth step, and E for the fifth and last step on the space
before each number.


A. Handling a Microscope
_____ 1. Inspect the parts if complete and functional.
_____ 2. Clean with tissue or cotton cloth the metal parts and lens paper for the lenses.
_____ 3. Put down gently the microscope about 2 inches away from the edge of the table.
_____ 4. Carry the microscope in an upright position to your working station.
_____ 5. Grab the arm with one hand and the base on the other hand.
B. Preparing a Wet Mount
_____ 1. Add a drop of water over the specimen (water act as a mounting medium).
_____ 2. Clean the microscope slide and cover slip.
_____ 3. Slowly drop the coverslip supported by a pin until it rests on the water and printed letter.
_____ 4. Position the cover slip 45o with one side touching one edge of the water.
_____ 5. Cut a small letter “e” from a newsprint and place it on the slide using a tweezer or forceps.
C. Setting Up a Microscope
_____ 1. Secure the slide using a stage clip and adjust the stage to center the specimen on the aperture.
_____ 2. Rotate the revolving nosepiece and set the LPO (a click sound is heard once the objective is in
position).
_____ 3. Turn on the illuminator or adjust the mirror for proper lightning (never use direct sunlight).
_____ 4. Raise the stage as you look at the side by turning the coarse adjustment knob.
_____ 5. Place the slide on the stage.
D. Focusing and Observing Specimen Under a Microscope
_____ 1. Turn the coarse adjustment knob upward to get a view of the specimen.
_____ 2. Look through the eyepiece with both eyes open.
_____ 3. Sharpen the focus by turning the fine adjustment knob.
_____ 4. Shift the objective by rotating the nosepiece to HPO for detailed view.
_____ 5. Move the slide up and down, side to side to get better image of the specimen.