Q. A: synthetic oligonucleotide polymers are used during annealing in a pcr

R: the primers bind to the double stranded dna at their complementary regions.

Which option is correct

Both are true

Both are false

A is true, R is false

A is false R is true

Answer। The correct option is 1: Both are true.

• Assertion (A): Synthetic oligonucleotide polymers (also known as primers) are indeed used during annealing in a PCR (Polymerase Chain Reaction). Their primary function is to bind to specific complementary sequences of DNA at the flanking regions of the target DNA fragment. This binding then allows the DNA polymerase to initiate DNA synthesis between the primers, amplifying the target DNA fragment during the subsequent cycles of PCR.
• Reason (R): The primers do bind to the double-stranded DNA at their complementary regions. This is crucial for their role in PCR. As mentioned earlier, the primers need to specifically match the flanking sequences of the target DNA to allow for efficient binding and subsequent DNA synthesis.
• Therefore, both the assertion and reason are true, and their connection is valid. The primers being used in annealing directly relate to their function of binding to the double-stranded DNA at their complementary regions.
• Other options are incorrect according to A and R statement।

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