1. What two structures on the microscope will you use to focus on your specimen?
2. Why should you never use the coarse adjustment knob on high power?
3. When do you use the fine adjustment knob?
4. Our microscopes have three objectives. What are their powers?
5. What is the magnification of the ocular lens?
6. What is the shortest objective called?
7. How do you switch objectives?
8. Which structure controls how much light passes through the specimen?
9. How should you carry the microscope?
10. How can you prevent your slide from slipping on the stage?
Answers & Comments
Answer:
1. Coarse Adjustment Knob & Fine Adjustment Knob
2. It will crack the slide
3.It moves the eyepiece up and down quickly, to get the thingy you're trying to look at roughly (or, ahem, “coarsely”), in focus.
4. Objective lenses come in various magnification powers, with the most common being 4x, 10x, 40x, and 100x, also known as scanning, low power, high power, and (typically) oil immersion objectives, respectively.
5. What is the magnifying power of the ocular lenses on your microscope? The magnifying power of the ocular lens is marked on the lens barrel (usually 10x). What is the magnifying power of the scanning lens on your microscope? Usually 4x.
6. After the light has passed through the specimen, it enters the objective lens (often called "objective" for short). The shortest of the three objectives is the scanning-power objective lens (N), and has a power of 4X.
7. Turn the revolving turret (2) so that the lowest power objective lens
8. Iris Diaphragm controls the amount of light reaching the specimen. It is located above the condenser and below the stage. Most high quality microscopes include an Abbe condenser with an iris diaphragm. Combined, they control both the focus and quantity of light applied to the specimen.
9. Important general rules: Always carry the microscope with 2 hands—place one hand on the microscope arm and the other hand under the microscope base. Do not touch the objective lenses (i.e. the tips of the objectives). Keep the objectives in the scan position and keep the stage low when adding or removing slides.
10. Use the stage clips. Is the image seen through the microscope oriented the same way as the object on the stage of the microscope